مشخصات فایل: قالب بندی: پاورپوینت ( قابل ویرایش ) تعداد اسلاید: 22 بخشی از پاورپوینت: DNA Extraction from primary sources may be complex Laboratory Needs Robust process, which can cope with a broad range of even viscous samples conditions Consistencyپاورپوینت بررسی سیستم های استخراج DNA|35015170|golden|بررسی سیستم های استخراج DNA, پاورپوینت بررسی سیستم های استخراج DNA, دانلود رایگان پاورپوینت بررسی سیستم های استخراج DNA, دانلود پاورپوینت بررسی سیستم های استخراج DNA, دانلود پاورپوینت در مورد بررسی سیستم های استخراج DNA, تحقیق بررسی سیستم های استخراج,,,
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مشخصات فایل:

قالب بندی: پاورپوینت ( قابل ویرایش )
تعداد اسلاید: 22

بخشی از پاورپوینت:

DNA Extraction from primary sources may be complex
Laboratory Needs
Robust process, which can cope with a broad range of even viscous samples conditions
Consistency in DNA yield & DNA quality
DNA should be used PCR, restrictions, cloning
Flexible hardware should allow downstream applications or foreign processes
Fully automated process
Expandable
Scalability
Laboratory Specs
DNA yield: up to 20µg–40µg (animal/plant)
DNA size: >30kBp
DNA Purity: 1.8-2.0 (OD260/280) PCR, Digests
Reproducibility: ~5% CV
Speed: ~192samples/2h (Offline Lysis)
Chemical Concepts
Beads (Magnetic / Silica)
Anion Exchange Chromatography
Organic Extractions & Precipitations
Contaminant Binding in Filter materials
Silica Filters
Automation Concepts
Vacuum Chambers
Magnetic Bead Separators
Semi-automated Approaches
Dedicated instruments
Liquid handling System & Automated Centrifuge
Current Status: Automated DNA extraction from complex samples
Vacuum filtration with limited robustness (clogging, cross-contamination)
Magnetic beads with limited performance (time consuming, liquid handling in viscous solution, carryover)
Contaminant binding with limited specificity & capacity (time consuming, centrifugation speed limited)
Dedicated instruments with limited applicability & flexibility
The Instrument
Features
Complete Integration (Including sample digestion)
Independent operation of centrifuge & liquid handling system
Modular Concept
Sample throughput 192/h
RCF up to 6.300g
Open to any chemistry
Basket Concept
Software
Applications
DNA extraction from animal tissues (mouse tails, mouse embryos, fish embryos, worms) [available]
DNA extraction from plant tissues (various plants and organs) [available]
Precipitation & Solubilisation Experiments
SPE
BAC DNA purification
Protein purification
Chemical Synthesis
Process for Animal Tissues I
Process for Animal Tissues II
Mouse Tails: DNA Yield & Size
Mouse Tails: Quality & Reproducibility
Cross Contamination Test
Plant Tissues: DNA Size & Yield
Plant Tissues: Digestibility
Plant Tissues: PCR
Achieved Technical Objectives
Robust application for DNA extraction from various complex sources were developed
DNA quality compatible for RT-PCR, restriction digests, southern & cloning
Fully Automated system for automated centrifugation, liquid handling, incubation and shaking
Liquid handling system and centrifuge can be used independently
Integrated multi-utility instrument
Achieved Lab Objectives
Reduced hands on time (load deck)
No human supervision necessary
Increased reproducibility
Modularity
Multipurpose Solution


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